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Difference between revisions of "Excel templ: Protocols"

From Bioblast
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This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
{| class="wikitable"
{| class="wikitable"
! Protocol name
! Protocol name
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! Step 19
! Step 19
! Step 20
! Step 20
! Short name
! Notes
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| Select protocol
| SUIT-001_O2_ce-pce_D003
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| RP1
| Doerrier 2018 Methods Mol Biol
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| SUIT-001_O2_PBMC-PLT_D004
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| SUIT-001 D001
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| Doerrier 2018 Methods Mol Biol
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| SUIT-001 D002
| SUIT-001_O2_pfi_D002
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| RP1
| Doerrier 2018 Methods Mol Biol
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| SUIT-002_O2_ce-pce-PBMC-PLT_D007a
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| RP2
| A: Doerrier 2018 Methods Mol Biol
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| SUIT-002_O2_ce-pce_D007
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| RP2
| A: Doerrier 2018 Methods Mol Biol
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| SUIT-002_O2_mt_D005
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| RP2
| A: Doerrier 2018 Methods Mol Biol
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| SUIT-002_O2_pfi_D006
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| RP2
| A: Doerrier 2018 Methods Mol Biol
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| SUIT-003_AmR_ce_D017
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_AmR_ce_D058
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_AmR_ce_D059
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce-pce_D013
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce-pce_D018
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce-pce_D020
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D009
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D012
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D028
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| CCP-ce
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| SUIT-003_O2_ce_D037
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D038
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| CCP-ce
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| SUIT-003_O2_ce_D039
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D050
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| CCP-ce
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| SUIT-003_O2_ce_D060
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| CCP-ce
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| SUIT-003_O2_ce_D061
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| SUIT-003_O2_ce_D062
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| CCP-ce
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| SUIT-004_O2_pfi_D010
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| RP1-short
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| SUIT-005_O2_pfi_D011
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| RP2-short
| A: when malate-anaplerotic activity is zero
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| SUIT-006_AmR_mt_D048
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_Fluo_mt_D034
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_Fluo_mt_D034_chemical background
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_MgG_mt_D055
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_O2_ce-pce_D029
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_O2_mt_D022
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-006_O2_mt_D047
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| CCP-mtprep
| A: Coupling control protocol, mtprep
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| SUIT-007_O2_ce-pce_D030
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| Glutamate anaplerosis
| A: Glutamate anaplerotic pathway
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| SUIT-008_O2_ce-pce_D025
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| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
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| SUIT-008_O2_mt_D026
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| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
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| SUIT-008_O2_pfi_D014
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| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
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| SUIT-009_AmR_ce-pce_D019
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| SUIT-009_AmR_mt_D021
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| SUIT-009_O2_ce-pce_D016
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| SUIT-009_O2_mt_D015
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| SUIT-010_O2_ce-pce_D008
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| Digitonin test
| A: Optimization of digitonin concentration for pce
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| SUIT-011_O2_pfi_D024
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| GM+S_OXPHOS+Rot_ET
| A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
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| SUIT-012_O2_ce-pce_D052
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| PM+G_OXPHOS
| A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
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| SUIT-012_O2_mt_D027
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| PM+G_OXPHOS
| A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
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| SUIT-013_AmR_ce_D023
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| GM+P+S_OXPHOS+Rot_ET
| A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
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| SUIT-015_O2_pti_D043
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| F+G+P+S_OXPHOS+Rot_ET
| A:  F-pathway in LEAK state and OXPHOS state
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| SUIT-016_O2_pfi_D044
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| F+G+S+Rot_OXPHOS+Omy
| A: F-pathway in LEAK state and OXPHOS state
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| SUIT-017_O2_mt _D046
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| F+G+S_OXPHOS+Rot_ET
| A:
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| SUIT-017_O2_pfi_D049
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| F+G+S_OXPHOS+Rot_ET
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| SUIT-018_AmR_mt_D031
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| Pal+Oct+P+G_OXPHOS+S+Rot_ET
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| SUIT-020_Fluo_mt_D033
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| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-020_Fluo_mt_D033_chemical_background
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| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-020_O2_mt_D032
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| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-021_Fluo_mt_D036
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| OXPHOS (GM+S+Rot+Omy)
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-021_Fluo_mt_D036_chemical background
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| OXPHOS (GM+S+Rot+Omy)
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-021_O2_mt_D035
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| OXPHOS (GM+S+Rot+Omy)
| A: simultaneous determination of O2 flux and mt-membrane potential
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| SUIT-022 O2 ce D051
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| AOX (ce CN+SHAM)
| A: Determination of the respiration due to the alternative oxidase pathway
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| SUIT-023 O2 ce D053
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| AOX-ce SHAM+CN
| A: Determination of respiration through the CIII-CIV pathway
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| SUIT-024_O2_ce-pce_D056
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| ATPase (PM)
| A: Determination of the presence of ATPases in mitochondrial preparations.
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| SUIT-001 D003
| SUIT-025_O2_mt_D057
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| OXPHOS (F+M+P+G+S+Rot)
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| SUIT-001 D004
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| SUIT-002 D005
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| SUIT-002 D006
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| Malate anaplerosis
| A: Malate anaplerotic pathway
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Revision as of 13:07, 8 July 2019

Attention! Attention!

Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.

This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.

Protocol name Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 Short name Notes
SUIT-001_O2_ce-pce_D003 RP1 Doerrier 2018 Methods Mol Biol
SUIT-001_O2_PBMC-PLT_D004 RP1 Doerrier 2018 Methods Mol Biol
SUIT-001_O2_mt_D001 1mt 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd RP1 Doerrier 2018 Methods Mol Biol
SUIT-001_O2_pfi_D002 RP1 Doerrier 2018 Methods Mol Biol
SUIT-002_O2_ce-pce-PBMC-PLT_D007a RP2 A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_ce-pce_D007 RP2 A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_mt_D005 RP2 A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_pfi_D006 RP2 A: Doerrier 2018 Methods Mol Biol
SUIT-003_AmR_ce_D017 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_AmR_ce_D058 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_AmR_ce_D059 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D013 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D018 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D020 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D009 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D012 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D028 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D037 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D038 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D039 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D050 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D060 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D061 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D062 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-004_O2_pfi_D010 RP1-short
SUIT-005_O2_pfi_D011 RP2-short A: when malate-anaplerotic activity is zero
SUIT-006_AmR_mt_D048 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_Fluo_mt_D034 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_Fluo_mt_D034_chemical background CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_MgG_mt_D055 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_O2_ce-pce_D029 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_O2_mt_D022 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-006_O2_mt_D047 CCP-mtprep A: Coupling control protocol, mtprep
SUIT-007_O2_ce-pce_D030 Glutamate anaplerosis A: Glutamate anaplerotic pathway
SUIT-008_O2_ce-pce_D025 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
SUIT-008_O2_mt_D026 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
SUIT-008_O2_pfi_D014 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
SUIT-009_AmR_ce-pce_D019
SUIT-009_AmR_mt_D021
SUIT-009_O2_ce-pce_D016
SUIT-009_O2_mt_D015
SUIT-010_O2_ce-pce_D008 Digitonin test A: Optimization of digitonin concentration for pce
SUIT-011_O2_pfi_D024 GM+S_OXPHOS+Rot_ET A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
SUIT-012_O2_ce-pce_D052 PM+G_OXPHOS A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-012_O2_mt_D027 PM+G_OXPHOS A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-013_AmR_ce_D023
SUIT-014_O2_pfi_D042 GM+P+S_OXPHOS+Rot_ET A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
SUIT-015_O2_pti_D043 F+G+P+S_OXPHOS+Rot_ET A: F-pathway in LEAK state and OXPHOS state
SUIT-016_O2_pfi_D044 F+G+S+Rot_OXPHOS+Omy A: F-pathway in LEAK state and OXPHOS state
SUIT-017_O2_mt _D046 F+G+S_OXPHOS+Rot_ET A:
SUIT-017_O2_pfi_D049 F+G+S_OXPHOS+Rot_ET A:
SUIT-018_AmR_mt_D031
SUIT-018_AmR_mt_D040
SUIT-018_AmR_mt_D041
SUIT-018_O2_mt_D054
SUIT-019_O2_pfi_D045 Pal+Oct+P+G_OXPHOS+S+Rot_ET A:
SUIT-020_Fluo_mt_D033 PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-020_Fluo_mt_D033_chemical_background PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-020_O2_mt_D032 PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_Fluo_mt_D036 OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_Fluo_mt_D036_chemical background OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_O2_mt_D035 OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-022 O2 ce D051 AOX (ce CN+SHAM) A: Determination of the respiration due to the alternative oxidase pathway
SUIT-023 O2 ce D053 AOX-ce SHAM+CN A: Determination of respiration through the CIII-CIV pathway
SUIT-024_O2_ce-pce_D056 ATPase (PM) A: Determination of the presence of ATPases in mitochondrial preparations.
SUIT-025_O2_mt_D057 OXPHOS (F+M+P+G+S+Rot)
SUIT-026_AmR_mt_D064
SUIT-026_O2_mt_D063
SUIT-027_O2_ce-pce_D065 Malate anaplerosis A: Malate anaplerotic pathway
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