ETS pathway types

high-resolution terminology - matching measurements at high-resolution

ETS pathway types

Description

Substrate types in SUIT protocols are types of reduced substrates feeding electrons into the electron transfer system (ETS) at different levels of mitochondrial pathways. Substrates of type 1 may be artificial electron donors essentially bypassing the ETS and feeding electrons directly into the terminal electron acceptor, cytochrome c oxidase (CIV) or alternative oxidases (single enzymatic step). Substrates of type 2 feed electrons into Complex III (CIII) with further electron transfer downstream of the Q-junction. Substrates of type 3 feed electrons into respiratory complexes directly upstream of the Q-junction, whereas substrates of type 4 feed electrons into dehydrogenases and enzyme systems upstream of the type 3 pathway level.

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MitoPedia topics: Substrate and metabolite

Contributed by Gnaiger E 2016-02-01

Substrate types on different pathway levels

Substrate types on the pathway level of converging NADH- and FADH₂-linked dehydrogenases, including the TCA cycle and beta-oxidation:

N: NADH-linked substrates (CI-linked)

F: FADH₂-linked substrates (FAO)

Substrate types on the pathway level of electron transfer complexes converging at the Q-junction:

S: Succinate (CII-linked)

Gp: Glycerophosphate (CGpDH-linked)

Substrate types on the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system:

Tm: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (As) and reducing cytochrome c as the substrate of CIV.