Deutschman 2019 J Cell Sci: Difference between revisions
(Created page with "{{Publication |title=Deutschman E, Ward JR, Kumar A, Ray G, Welch N, Lemieux ME, Dasarathy S, Longworth MS (2019) Condensin II protein dysfunction impacts mitochondrial respir...") ย |
No edit summary ย |
||
(2 intermediate revisions by the same user not shown) | |||
Line 10: | Line 10: | ||
|keywords=Condensin, Mitochondria, Oxidative stress | |keywords=Condensin, Mitochondria, Oxidative stress | ||
|editor=[[Plangger M]], | |editor=[[Plangger M]], | ||
|mipnetlab=US OH Cleveland Dasarathy S | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Genetic knockout;overexpression | ||
|injuries=Oxidative stress;RONS | |||
|organism=Human | |||
|tissues=Endothelial;epithelial;mesothelial cell | |||
|preparations=Permeabilized cells | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, CIV, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2019-11, | |additional=Labels, 2019-11, | ||
}} | }} |
Latest revision as of 15:44, 27 November 2019
Deutschman E, Ward JR, Kumar A, Ray G, Welch N, Lemieux ME, Dasarathy S, Longworth MS (2019) Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses. J Cell Sci 132:jcs233783. |
Deutschman E, Ward JR, Kumar A, Ray G, Welch N, Lemieux ME, Dasarathy S, Longworth MS (2019) J Cell Sci
Abstract: The maintenance of mitochondrial respiratory function and homeostasis is essential to human health. Here, we identify condensin II subunits as novel regulators of mitochondrial respiration and mitochondrial stress responses. Condensin II is present in the nucleus and cytoplasm. While the effects of condensin II depletion on nuclear genome organization are well studied, the effects on essential cytoplasmic and metabolic processes are not as well understood. Excitingly, we observe that condensin II chromosome-associated protein (CAP) subunits individually localize to different regions of mitochondria, suggesting possible mitochondrial-specific functions independent from those mediated by the canonical condensin II holocomplex. Changes in cellular ATP levels and mitochondrial respiration are observed in condensin II CAP subunit-deficient cells. Surprisingly, we find that loss of NCAPD3 also sensitizes cells to oxidative stress. Together, these studies identify new, and possibly independent, roles for condensin II CAP subunits in preventing mitochondrial damage and dysfunction. These findings reveal a new area of condensin protein research that could contribute to the identification of targets to treat diseases where aberrant function of condensin II proteins is implicated.
ยฉ 2019. Published by The Company of Biologists Ltd. โข Keywords: Condensin, Mitochondria, Oxidative stress โข Bioblast editor: Plangger M โข O2k-Network Lab: US OH Cleveland Dasarathy S
Labels: MiParea: Respiration, Genetic knockout;overexpression
Stress:Oxidative stress;RONS Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
Labels, 2019-11