De Araujo 2015 Cold Spring Harb Protoc-C
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| de Araújo ME, Lamberti G, Huber LA (2015) Purification of Early and Late Endosomes. Cold Spring Harb Protoc 2015(12):pdb.top074443. |
» http://www.ncbi.nlm.nih.gov/pubmed/26631131
de Araújo ME, Lamberti G, Huber LA (2015) Cold Spring Harb Protoc
Abstract: Proteomic analysis of early and late endosomes has been constrained by the limited purity of the endosomal fractions that can be achieved by biochemical methods. Here we briefly review endocytic pathways, and then introduce fractionation strategies that have been used to improve the purity of isolated endosomes. In addition, we describe innovative proteomics analysis methods that have been shown to partially circumvent the limitations found in the enrichment steps.
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