Difference between revisions of "Cyanide"
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=== Preparation of KCN stock === | === Preparation of KCN stock === | ||
:::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; | :::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; M = 65.12 g·mol<sup>-1</sup>. | ||
:::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! </span> | :::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! </span> |
Revision as of 12:49, 12 February 2021
Description
Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Last update 2021-01-22
Inhibitor titrations, CIV-assay, alternative oxidase and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
- alternative oxidase
- Caution: Due to a confusion, a change in the procedure for preparing the KCN was done in April 2019 that recommended to prepare 20 mM KCN stock and titrate 100 µL. This now (2021-01-22) has been corrected to our previous recommendation of 1 M KCN stock solutions
Preparation of KCN stock
- Kcn(KCN): Fluka 60178, 100 g, store at R.T.; M = 65.12 g·mol-1.
- Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic!
- Preparation of 0.5 M KCN stock solution (dissolved in H2O):
- Weigh 32.6 mg of KCN.
- Dissolve in 1 ml H2O.
- The pH of the solution is basic. Since the titration amount is low and HCN may evaporate during pH adjustment with HCl, the pH is not adjusted.
- Divide into 0.2 mL portions
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 1 mM.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
MitoPedia topics: Inhibitor