Difference between revisions of "Coupling Quiz"

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|type="()"}

- After addition of kinetically saturation doses of ADP

|| The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and P''i''.

|| The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi.

- After ~~uncoupler~~ titration

- After protonophore titration

|| ~~Correct~~ answer is ~~the '''[[charge number]]'''. '''[[Charge number]]'''~~ of an ion ~~or electrochemical reaction is~~ the ~~[[charge]] divided by the [[elementary charge]]~~ of ~~the ion or~~ of ~~electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''~~B ~~= ''Q''~~B·''e''~~~~-~~1; [[Cohen 2008 IUPAC Green Book]]~~

|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. The intrinsic proton leak is the ''uncoupled'' leak current of protons in which protons diffuse across the mtIM in the dissipative direction of the downhill protonmotive force without coupling to phosphorylation. Protonophores are ''uncouplers'' which are titrated to obtain maximum ''noncoupled'' respiration as a measure of ET-capacity.

- In the absence of substrates

|| ~~Correct~~ answer ~~is the '''[[charge number]]'''~~. ~~'''[[Charge number]]''' of an ion or electrochemical reaction~~ is ~~the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction~~ as ~~defined in the reaction equation. ''z'' is~~ a ~~positive integer. ''z''~~B ~~= ''Q''~~B~~·''e''~~<~~sup~~>-1</~~sup~~>~~; [[Cohen 2008 IUPAC Green Book]]~~

|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. Therefore substrates are necessary to reach LEAK state of mitochondrial respiration.

+ In the absence of ADP

|| Correct ~~answer~~ is ~~the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction~~ as ~~defined in the reaction equation. ''z'' is~~ a ~~positive integer. ''z''~~B ~~= ''Q''~~B~~·''e''~~<~~sup~~>-1</~~sup~~>~~; [[Cohen 2008 IUPAC Green Book]]~~

|| Correct! The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. In the absence of ADP no ATP synthesis can procede and we measure low, cation leak-dependent respiration.

{How are the units of electric energy [J] and electric force [V] related?

	V = J/C
	V = J·C
	V = (J·F)/C
	V = J/(C·F)

	-1.2 V
	- 120 V
	- 1.2 kV
	1.2 V

	To compliment our brain mitochondria
	To get free drinks
	To feel insecure
	To express both in identical motive units [MU]

Difference between revisions of "Coupling Quiz"

Latest revision as of 12:36, 27 January 2020

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List of Quizzes on Bioblast

@@ Line 16: / Line 16: @@
 |type="()"}
 - After addition of kinetically saturation doses of ADP
-|| The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>''i''</sub>.
+|| The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>.
-- After uncoupler titration
+- After protonophore titration
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. The intrinsic proton leak is the ''uncoupled'' leak current of protons in which protons diffuse across the mtIM in the dissipative direction of the downhill protonmotive force without coupling to phosphorylation. Protonophores are ''uncouplers'' which are titrated to obtain maximum ''noncoupled'' respiration as a measure of ET-capacity.
 - In the absence of substrates
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. Therefore substrates are necessary to reach LEAK state of mitochondrial respiration.
 + In the absence of ADP
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Correct! The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. In the absence of ADP no ATP synthesis can procede and we measure low, cation leak-dependent respiration.
 {How are the units of electric energy [J] and electric force [V] related?

	In the absence of substrates
	After protonophore titration
	After addition of kinetically saturation doses of ADP
	In the absence of ADP