Difference between revisions of "Coupling Quiz"

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|type="{}"}

{ ET } state.

|| Compared to OXPHOS-capacity, the oxidative ET-capacity reveals the limitation of ~~OXPHOScapacity~~ mediated by the phosphorylation-pathway. By application of external uncouplers, ~~ETcapacity~~ is measured as noncoupled respiration.

|| Compared to OXPHOS-capacity, the oxidative ET-capacity reveals the limitation of OXPHOS-capacity mediated by the phosphorylation-pathway. By application of external uncouplers, ET-capacity is measured as noncoupled respiration.

{LEAK state can be measured:

{Convert the molar format of the Gibbs force of reaction, Δk''F''''n''O2 [kJ/mol], into the electrical format, Δk''F''''e''O2 [V]. Which physicochemical constant is required?

~~|type="{}"}~~

~~{ Faraday } constant~~

|| The '''Faraday constant''', ''F'', [C/mol]. Links the electric charge [C] to amount [mol], and thus relates the [[electrical format]], ''e'' [C], to the [[molar format]], ''n'' [mol]. The Farady constant, ''F'' = ''e''·''N''A = 96,485.33 C/mol, is the product of [[elementary charge]], ''e'' = 1.602176634∙10-19 C/x, and the [[Avogadro constant]], ''N''A = 6.02214076∙1023 x/mol. The dimensionless unit [x] is not explicitely considered by IUPAC. More info https:~~//www.bipm.org/en/measurement-units/rev-si/~~

~~{What is the meaning of the symbol ''z''O2?~~

|type="()"}

~~+ [[Charge number]]~~ of O2 ~~= 4~~

- After addition of kinetically saturation doses of ADP

~~|| The '''[[charge number]]''' of an~~ ion ~~or electrochemical reaction is~~ the ~~[[charge]] divided by the [[elementary charge]]~~ of ~~the ion or~~ of ~~electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''~~B ~~= ''Q''~~B~~·''e''~~-~~1; [[Cohen 2008 IUPAC Green Book]]~~

|| The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi.

- ~~[[Atomic number]]~~ of O2 ~~= 8~~

- After protonophore titration

~~|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an~~ ion ~~or electrochemical reaction is~~ the ~~[[charge]] divided by the [[elementary charge]]~~ of ~~the ion or~~ of ~~electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''~~B ~~= ''Q''~~B·''e''~~-1; [[Cohen 2008 IUPAC Green Book]]~~

|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. The intrinsic proton leak is the ''uncoupled'' leak current of protons in which protons diffuse across the mtIM in the dissipative direction of the downhill protonmotive force without coupling to phosphorylation. Protonophores are ''uncouplers'' which are titrated to obtain maximum ''noncoupled'' respiration as a measure of ET-capacity.

~~- [[Elementary charge]]~~ of ~~O2~~ in ~~[C]~~

- In the absence of substrates

~~|| Correct answer is~~ the '''~~[[charge number]]~~'''~~. '''[[Charge number]]'~~'' of ~~an ion or electrochemical reaction is~~ the ~~[[charge]] divided by the [[elementary charge]]~~ of ~~the ion or of electrons transferred in the reaction as defined in the reaction equation~~. ~~''z''~~ is a ~~positive integer. ''z''~~B ~~= ''Q''~~B~~·''e''~~<~~sup~~>-1</~~sup~~>~~; [[Cohen 2008 IUPAC Green Book]]~~

|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. Therefore substrates are necessary to reach LEAK state of mitochondrial respiration.

- ~~Alphabetical order~~ of O2 ~~isotope~~

+ In the absence of ADP

~~|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an~~ ion ~~or electrochemical reaction is~~ the ~~[[charge]] divided by the [[elementary charge]]~~ of ~~the ion or~~ of ~~electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''~~B ~~= ''Q''~~B~~·''e''~~-~~1; [[Cohen 2008 IUPAC Green Book]]~~

|| Correct! The LEAK-state is defined as a state of mitochondrial respiration when O2 flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O2, respiratory fuel substrates and Pi. In the absence of ADP no ATP synthesis can procede and we measure low, cation leak-dependent respiration.

{How are the units of electric energy [J] and electric force [V] related?

	V = J/(C·F)
	V = (J·F)/C
	V = J/C
	V = J·C

	1.2 V
	-1.2 V
	- 120 V
	- 1.2 kV

	To get free drinks
	To express both in identical motive units [MU]
	To feel insecure
	To compliment our brain mitochondria

Difference between revisions of "Coupling Quiz"

Latest revision as of 12:36, 27 January 2020

Do you feel the Coupling?

List of Quizzes on Bioblast

@@ Line 10: / Line 10: @@
 |type="{}"}
 { ET } state.
-||  Compared to OXPHOS-capacity, the oxidative ET-capacity reveals the limitation of OXPHOScapacity mediated by the phosphorylation-pathway. By application of external uncouplers, ETcapacity is measured as noncoupled respiration.
+||  Compared to OXPHOS-capacity, the oxidative ET-capacity reveals the limitation of OXPHOS-capacity mediated by the phosphorylation-pathway. By application of external uncouplers, ET-capacity is measured as noncoupled respiration.
+{LEAK state can be measured:
-{Convert the molar format of the Gibbs force of reaction, Δ<sub>k</sub>''F''<sub><u>''n''</u>O2</sub> [kJ/mol], into the electrical format, Δ<sub>k</sub>''F''<sub><u>''e''</u>O2</sub> [V]. Which physicochemical constant is required?
-|type="{}"}
-{ Faraday } constant
-|| The '''Faraday constant''', ''F'', [C/mol]. Links the electric charge [C] to amount [mol], and thus relates the [[electrical format]], <u>''e''</u> [C], to the [[molar format]], <u>''n''</u> [mol]. The Farady constant, ''F'' = ''e''·''N''<sub>A</sub> = 96,485.33 C/mol, is the product of [[elementary charge]], ''e'' = 1.602176634∙10<sup>-19</sup> C/x, and the [[Avogadro constant]], ''N''<sub>A</sub> = 6.02214076∙10<sup>23</sup> x/mol. The dimensionless unit [x] is not explicitely considered by IUPAC. More info https://www.bipm.org/en/measurement-units/rev-si/
-{What is the meaning of the symbol ''z''<sub>O2</sub>?
 |type="()"}
-+ [[Charge number]] of O<sub>2</sub> = 4
+- After addition of kinetically saturation doses of ADP
-|| The '''[[charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>.
-- [[Atomic number]] of O<sub>2</sub> = 8
+- After protonophore titration
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. The intrinsic proton leak is the ''uncoupled'' leak current of protons in which protons diffuse across the mtIM in the dissipative direction of the downhill protonmotive force without coupling to phosphorylation. Protonophores are ''uncouplers'' which are titrated to obtain maximum ''noncoupled'' respiration as a measure of ET-capacity.
-- [[Elementary charge]] of O<sub>2</sub> in [C]
+- In the absence of substrates
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. Therefore substrates are necessary to reach LEAK state of mitochondrial respiration.
-- Alphabetical order of O<sub>2</sub> isotope
++ In the absence of ADP
-|| Correct answer is the '''[[charge number]]'''. '''[[Charge number]]''' of an ion or electrochemical reaction is the [[charge]] divided by the [[elementary charge]] of the ion or of electrons transferred in the reaction as defined in the reaction equation. ''z'' is a positive integer. ''z''<sub>B</sub> = ''Q''<sub>B</sub>·''e''<sup>-1</sup>; [[Cohen 2008 IUPAC Green Book]]
+|| Correct! The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. In the absence of ADP no ATP synthesis can procede and we measure low, cation leak-dependent respiration.
 {How are the units of electric energy [J] and electric force [V] related?

	After addition of kinetically saturation doses of ADP
	In the absence of ADP
	After protonophore titration
	In the absence of substrates