Coupling-control protocol: Difference between revisions
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Revision as of 12:26, 31 May 2011
- high-resolution terminology - matching measurements at high-resolution
Coupling-control protocol
Description
A coupling control protocol induces different coupling control states at constant substrate supply. In intact cells, CCP can be appled by using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. FCCP). Coupling control states in intact cells include R, L, E; LEAK, ROUTINE, and ETS. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include L, P, E; LEAK, OXPHOS, and ETS.
Abbreviation: CCP
Reference: Pesta_2011_Protocols
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
