Chicco 2022 MitoFit: Difference between revisions
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[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | |||
{{MitoFit page name}} | {{MitoFit page name}} | ||
{{Publication | {{Publication | ||
|title= | |title=Chicco AJ, Zilhaver PT, Whitcomb LA, Fresa KJ, Izon CS, Gonzalez-Franquesa A, Izon CS, Dometita C, Irving BA, Garcia-Roves PM (2022) Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols. https://doi.org/10.26124/mitofit:2022-0017 | ||
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=|MitoFit pdf]] [ Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols | |info=MitoFit Preprints 2022.17. [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/3/33/Chicco_2022_MitoFit.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/3/33/Chicco_2022_MitoFit.pdf Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols] [[File:WatchThePresentationYoutube_icon.jpg|200px|link=https://www.youtube.com/watch?v=mNSu-hY3hUg&t=1001s|»''Watch the presentation''«]]<br/> | ||
|authors=Chicco | |authors=Chicco Adam J, Zilhaver Philip T, Whitcomb Luke A, Fresa Kyle J, Izon Cheyanne S, Gonzalez-Franquesa Alba, Dometita Crystal, Irving Brian A, Garcia-Roves Pablo Miguel | ||
|year=2022 | |||
|journal=MitoFit Prep | |||
|abstract=[[Chicco 2022 Abstract Bioblast]]: Multi-substrate respirometry protocols are frequently used to resolve the relative contributions of NADH-producing (N-pathway or CI-linked) substrates and succinate (S-pathway or CII-linked substrate) to mitochondrial oxygen consumption rate (''J''<sub>O<sub>2</sub></sub>). Similarly, rotenone (a selective CI inhibitor) is utilized in the presence of N+S substrates to deduce the contribution of N-pathway flux to the total (NS-pathway) ''J''<sub>O<sub>2</sub></sub>. However, under S- and some NS-pathway states, rotenone elicits a paradoxical increase in ''J''<sub>O<sub>2</sub></sub>, revealing a complex interaction of N- and S-pathway substrate oxidation on ''J''<sub>O<sub>2</sub></sub> ''in vitro''. Herein, we demonstrate inhibitory effects of >1 mM malate or malonate (a CII inhibitor) on ''J''<sub>O<sub>2</sub></sub> supported by pyruvate and/or glutamate, suggesting that endogenous succinate oxidation interacts with malate concentration to potently regulate ''J''<sub>O<sub>2</sub></sub> supported by N-pathway substrates in a tissue-specific manner. Potential mechanisms are discussed to stimulate further experimentation aimed at elucidating the biological bases for variations in NS-pathway flux in multi-substrate respirometry protocols. | |||
|keywords=Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry | |keywords=Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry | ||
|editor=Tindle-Solomon L | |editor=Tindle-Solomon L | ||
|mipnetlab=US CO Fort Collins Chicco AJ, US LA Baton Rouge Irving BA, ES Barcelona Garcia-Roves PM | |||
}} | }} | ||
== Discussion == | |||
See [[Talk:Chicco 2022 MitoFit]] | |||
{{Labeling | {{Labeling | ||
|area= | |area=Respiration | ||
| | |preparations=Isolated mitochondria | ||
| | |pathways=N, S, NS | ||
|instruments=Oxygraph-2k | |||
|additional=Bioblast 2022 | |additional=Bioblast 2022 | ||
}} | }} | ||
Latest revision as of 07:52, 8 January 2023
Chicco 2022 MitoFit
| Chicco AJ, Zilhaver PT, Whitcomb LA, Fresa KJ, Izon CS, Gonzalez-Franquesa A, Izon CS, Dometita C, Irving BA, Garcia-Roves PM (2022) Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols. https://doi.org/10.26124/mitofit:2022-0017 |
» MitoFit Preprints 2022.17.
Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols 
Chicco Adam J, Zilhaver Philip T, Whitcomb Luke A, Fresa Kyle J, Izon Cheyanne S, Gonzalez-Franquesa Alba, Dometita Crystal, Irving Brian A, Garcia-Roves Pablo Miguel (2022) MitoFit Prep
Abstract: Chicco 2022 Abstract Bioblast: Multi-substrate respirometry protocols are frequently used to resolve the relative contributions of NADH-producing (N-pathway or CI-linked) substrates and succinate (S-pathway or CII-linked substrate) to mitochondrial oxygen consumption rate (JO2). Similarly, rotenone (a selective CI inhibitor) is utilized in the presence of N+S substrates to deduce the contribution of N-pathway flux to the total (NS-pathway) JO2. However, under S- and some NS-pathway states, rotenone elicits a paradoxical increase in JO2, revealing a complex interaction of N- and S-pathway substrate oxidation on JO2 in vitro. Herein, we demonstrate inhibitory effects of >1 mM malate or malonate (a CII inhibitor) on JO2 supported by pyruvate and/or glutamate, suggesting that endogenous succinate oxidation interacts with malate concentration to potently regulate JO2 supported by N-pathway substrates in a tissue-specific manner. Potential mechanisms are discussed to stimulate further experimentation aimed at elucidating the biological bases for variations in NS-pathway flux in multi-substrate respirometry protocols. • Keywords: Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry • Bioblast editor: Tindle-Solomon L • O2k-Network Lab: US CO Fort Collins Chicco AJ, US LA Baton Rouge Irving BA, ES Barcelona Garcia-Roves PM
Discussion
See Talk:Chicco 2022 MitoFit
Labels: MiParea: Respiration
Preparation: Isolated mitochondria
Pathway: N, S, NS HRR: Oxygraph-2k
Bioblast 2022
