Carnitine: Difference between revisions

Revision as of 10:48, 22 March 2021

high-resolution terminology - matching measurements at high-resolution

Carnitine

Description

Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.

Abbreviation: Car

Application in HRR

Car: L-carnitine: Sigma Aldrich; C0158; M = 161.2 g·mol^-1

Preparation of 250 mM stock solution (dissolved in H₂O)

Weigh 100 mg of Carnitine.
Add 2.48 mL H₂O.
Divide into 100 µL portions.
Store frozen at -20°C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
Final concentration: 0.5 mM.

Feeding carnitine

MitoPedia topics: Substrate and metabolite

@@ Line 6: / Line 6: @@
 == Application in [[HRR]] ==
 ::::* Car:  '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 g·mol<sup>-1</sup>
-::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O):'''
+:::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O)
 ::::# Weigh 100 mg of Carnitine.
 ::::# Add 2.48 mL H<sub>2</sub>O.
@@ Line 12: / Line 15: @@
 ::::# Store frozen at -20°C.
-:::: '''O2k Manual titration'''
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: 4 µL 250 mM stock using a 10 µL Hamilton syringe (2 mL O2k-Chamber).
+::::* Titration volume ('''2-mL O2k-chamber'''): 4 µL using a 10 µL Hamilton syringe.
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe
 ::::* Final concentration: 0.5 mM.