Carboxyatractyloside: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Cat: Carboxyatractyloside''' ( | '''Cat: Carboxyatractyloside''' (carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); '''Alternative source''': Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>43</sub>O<sub>18</sub>S<sub>2</sub>K<sub>3</sub>*3H<sub>2</sub>O), 5 mg, store at -20 °C, FW = 939.1). | ||
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'''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0): | '''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0): | ||
::1) | ::1) Weigh 4.7 mg carboxyatractyloside. | ||
::2) | ::2) Dissolve in 1 ml H<sub>2</sub>O. | ||
::3) | ::3) Divide into 0.2 ml aliquots. | ||
::4) | ::4) Store at -20 °C. | ||
Revision as of 15:53, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Carboxyatractyloside
Description
Carboxyatractyloside is a highly selective and potent inhibitor of the Adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: Cat
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Cat: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 °C, FW = 939.1).
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!
Preparation of 5 mM stock solution (dissolved in H20):
- 1) Weigh 4.7 mg carboxyatractyloside.
- 2) Dissolve in 1 ml H2O.
- 3) Divide into 0.2 ml aliquots.
- 4) Store at -20 °C.
Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.
* Talk:Carboxyatractyloside
