Brewer 2004 Mitochondrion: Difference between revisions
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{{Publication | {{Publication | ||
|title=Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4: 49-57. | |title=Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4:49-57. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16120374 PMID: 16120374] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/16120374 PMID: 16120374] | ||
|authors=Brewer GJ, Jones TT, Wallimann T, Schlattner U | |authors=Brewer GJ, Jones TT, Wallimann T, Schlattner U | ||
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|abstract=We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA±SCAVEGR™ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. | |abstract=We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA±SCAVEGR™ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. | ||
|keywords=Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS | |keywords=Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS | ||
|mipnetlab= | |mipnetlab=CH Zurich Wallimann T, US IL Springfield Brewer GJ, FR Grenoble Schlattner U | ||
}} | }} | ||
== Cited by == | |||
{{Template:Cited by Gnaiger 2020 BEC MitoPathways}} | |||
{{Labeling | {{Labeling | ||
|injuries=Oxidative stress;RONS | |||
|organism=Rat | |||
|tissues=Nervous system | |||
|preparations=Isolated mitochondria | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |additional=BEC 2020.2 | ||
}} | }} | ||
Latest revision as of 16:55, 16 January 2021
| Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4:49-57. |
Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Mitochondrion
Abstract: We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA±SCAVEGR™ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. • Keywords: Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS
• O2k-Network Lab: CH Zurich Wallimann T, US IL Springfield Brewer GJ, FR Grenoble Schlattner U
Cited by
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria
HRR: Oxygraph-2k
BEC 2020.2
