Beryllium sulfate: Difference between revisions
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | {{Chemical_description | ||
|abbr=BeS | |abbr=BeS | ||
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|complete name=Beryllium sulfate | |complete name=Beryllium sulfate | ||
|chem formula=BeSO<sub>4</sub> | |chem formula=BeSO<sub>4</sub> | ||
|molar mass= | |molar mass= 177.14 | ||
|vendor= | |vendor= | ||
|product number= | |product number= | ||
|store at= | |store at=4 °C | ||
|sensitivity= | |sensitivity= | ||
|cas= | |cas= | ||
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|h info= | |h info= | ||
}} | }} | ||
:::: '''Preparation of 200 mM stock solution''' (dissolved in H<sub>2</sub>O) | :::: '''Preparation of 200 mM stock solution''' (dissolved in H<sub>2</sub>O) | ||
::::# Weigh 354.3 mg. | |||
::::# Dissolve in 10 mL distilled H2O under a fume hood. | |||
::::# Divide in aliquots. | |||
::::# Store at +4 °C; | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 2 µL using a 10 µL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 200 µM. | |||
== References == | == References == | ||
::::* [ | ::::* [[Chinopoulos 2014 Methods Enzymol]] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
Latest revision as of 12:57, 26 April 2024
- high-resolution terminology - matching measurements at high-resolution
Beryllium sulfate
Description
Beryllium sulfate is used in combination with sodium fluoride to form beryllium trifluoride (BeF3−), to inhibit the ATP synthase if it is exposed by disruption of the mitochondrial membranes.
Abbreviation: BeS
Application in HRR
- BeS: (Beryllium sulfate; BeSO4), store at 4 °C, M = 177.14 g·mol-1
- Preparation of 200 mM stock solution (dissolved in H2O)
- Weigh 354.3 mg.
- Dissolve in 10 mL distilled H2O under a fume hood.
- Divide in aliquots.
- Store at +4 °C;
- Preparation of 200 mM stock solution (dissolved in H2O)
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 µL using a 10 µL syringe (2 mL O2k-chamber).
- Final concentration: 200 µM.
References
MitoPedia topics: Inhibitor
