Assay: Difference between revisions
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|abbr=n.a. | |abbr=n.a. | ||
|description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. | |description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An [[assay series]] consists of replications of ''N'' assays on different samples. | ||
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{{MitoPedia methods | {{MitoPedia methods |
Revision as of 07:43, 27 January 2016
Description
An experimental assay is a test run on a single sample. An assay may have n repetitions for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An assay series consists of replications of N assays on different samples.
Abbreviation: n.a.
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
MitoPedia topics: Sample preparation