Difference between revisions of "Ap5A"
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|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | ||
}} | |||
__TOC__ | |||
== Application in [[HRR]] == | |||
{{Chemical_description | |||
|abbr=Ap5A | |||
|trivial name=Ap5A | |||
|complete name=P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt | |||
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> · 5 Na | |||
|molar mass=1026.28 | |||
|vendor=Sigma-Aldrich | |||
|product number=D4022 | |||
|store at=-20 °C | |||
|sensitivity= | |||
|cas=4097-04-5 | |||
|h statements= | |||
|h info= | |||
}}<!--:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | |||
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>--> | |||
::: '''Preparation of 100 mM stock solution''' (dissolved in | :::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O) | ||
::::# | ::::# Dissolve content of Sigma 2 mg glass vial in 19.49 µL H<sub>2</sub>O | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe. | |||
::: '''O2k | |||
::::* Final concentration: 50 µM. | ::::* Final concentration: 50 µM. | ||
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::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} |
Latest revision as of 10:25, 21 June 2021
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Abbreviation: Ap5A
Application in HRR
- Ap5A: Ap5A (P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt; C20H29N10O22P5 · 5 Na), Sigma-Aldrich: D4022, store at -20 °C, CAS: 4097-04-5, M = 1026.28 g·mol-1
- Preparation of 100 mM stock solution (dissolved in H2O)
- Dissolve content of Sigma 2 mg glass vial in 19.49 µL H2O
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 50 µM.
References
MitoPedia topics: Inhibitor