Difference between revisions of "Ap5A"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | |||
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | {{Chemical_description | ||
|abbr=Ap5a | |||
|trivial name=P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt | |||
|complete name= | |||
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> · 5 Na | |||
|molar mass=1026.28 | |||
|vendor=Sigma-Aldrich | |||
|product number=D4022 | |||
|store at=-20 °C | |||
|sensitivity= | |||
|cas=4097-04-5 | |||
|h statements= | |||
|h info= | |||
}}<!--:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | |||
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>--> | |||
Revision as of 12:03, 11 June 2021
- high-resolution terminology - matching measurements at high-resolution
Ap5A
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Abbreviation: Ap5A
Application in HRR
- Ap5a: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt ( C20H29N10O22P5 · 5 Na), Sigma-Aldrich: D4022, store at -20 °C, CAS: 4097-04-5, M = 1026.28 g·mol-1
- Preparation of 100 mM stock solution (dissolved in H2O)
- Dissolve content of Sigma 2 mg glass vial in 19.49 µL H2O
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 50 µM.
References
MitoPedia topics: Inhibitor
