Difference between revisions of "Additive effect of convergent electron flow"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''A''<sub>''α+β''</sub> | |abbr=''A''<sub>''α+β''</sub> | ||
|description='''Additivity''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of | |description='''Additivity''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of convergent CI+II electron flow''' is a consequence of electron flow converging at the '''Q-junction''' from respiratory Complexes I and II ([[CI+II e-input]]). Further additivity may be observed by convergent electron flow through [[glycerophosphate dehydrogenase]] and [[electron-transferring flavoprotein]]. Convergent electron flow corresponds to the operation of the [[TCA cycle]] and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the [[Q-junction]] supports higher [[OXPHOS capacity]] and [[ETS capacity]] than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular [[TCA cycle]] function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[Mitochondrial preparations|mt-preparations]] have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to [[additivity]]. | ||
|info=[[MiPNet12.12]], [[Gnaiger_2009_Int J Biochem Cell Biol]] | |info=[[MiPNet12.12]], [[Gnaiger_2009_Int J Biochem Cell Biol]] | ||
|type=Respiration | |type=Respiration |
Revision as of 19:33, 25 July 2012
- high-resolution terminology - matching measurements at high-resolution
Additive effect of convergent electron flow
Description
Additivity describes the princple of substrate control of mitochondrial respiration, where the additive effect of convergent CI+II electron flow is a consequence of electron flow converging at the Q-junction from respiratory Complexes I and II (CI+II e-input). Further additivity may be observed by convergent electron flow through glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the Q-junction supports higher OXPHOS capacity and ETS capacity than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to additivity.
Abbreviation: Aα+β
Reference: MiPNet12.12, Gnaiger_2009_Int J Biochem Cell Biol
MitoPedia methods:
Respirometry
MitoPedia topics:
Substrate and metabolite