Difference between revisions of "Additive effect of convergent electron flow"

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Additive effect of convergent electron flow

Description

Additivity describes the princple of substrate control of mitochondrial respiration, where the additive effect of convertent CI+II electron flow is a consequence of electron flow converging at the Q-junction from respiratory Complexes I and II (CI+II e-input). Further additivity may be observed by convergent electron flow through glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the Q-junction supports higher OXPHOS capacity and ETS capacity than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to additivity.

Abbreviation: A_α+β

Reference: MiPNet12.12, Gnaiger_2009_Int J Biochem Cell Biol

MitoPedia methods: Respirometry

MitoPedia topics: Substrate and metabolite

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 {{MitoPedia
 |abbr=''A''<sub>''α+β''</sub>
-|description='''Additivitiy''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of convertent CI+II electron flow''' is a consequence of electron flow converging at the '''Q-junction''' from respiratory Complexes I and II ([[CI+II e-input]]).  Further additivity may be observed by convergent electron flow through [[glycerophosphate dehydrogenase]] and [[electron-transferring flavoprotein]].  Convergent electron flow corresponds to the operation of the [[TCA cycle]] and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the [[Q-junction]] supports higher [[OXPHOS capacity]] and [[ETS capacity]] than separate electron flow through either CI or CII.  Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular [[TCA cycle]] function.  The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[mt-preparations]] have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to [[additivity]].
+|description='''Additivity''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of convertent CI+II electron flow''' is a consequence of electron flow converging at the '''Q-junction''' from respiratory Complexes I and II ([[CI+II e-input]]).  Further additivity may be observed by convergent electron flow through [[glycerophosphate dehydrogenase]] and [[electron-transferring flavoprotein]].  Convergent electron flow corresponds to the operation of the [[TCA cycle]] and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the [[Q-junction]] supports higher [[OXPHOS capacity]] and [[ETS capacity]] than separate electron flow through either CI or CII.  Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular [[TCA cycle]] function.  The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[mt-preparations]] have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to [[additivity]].
 |info=[[MiPNet12.12]], [[Gnaiger_2009_Int J Biochem Cell Biol]]
 |type=Respiration