2-Deoxyglucose: Difference between revisions
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::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): ย | ::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): ย | ||
::::# weigh | ::::# weigh 1 g of 2-deoxy-D-glucose | ||
::::# dissolve 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | ::::# dissolve 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | ||
Revision as of 11:15, 21 June 2021
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
Abbreviation: 2-DG
Reference: Proton_flux, O2k-pH_ISE-Module
Application in HRR
- 2-DG: 2-deoxy-D-glucose C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 ยฐC; M = 164.16 gยทmol-1
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 1 g of 2-deoxy-D-glucose
- dissolve 3.0458 mL H2O in a 10 mL-glass beaker
- ยปO2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 25 ยตL using a 50 ยตL syringe
- Final conc. in 2 mL O2k-chamber: 25 mM
MitoPedia topics:
Inhibitor