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Meszaros 2017 MiPschool Obergurgl
Andras Meszaros

(Very) High-resolution respirometry – a deep dive into oxygen dependence of mitochondrial respiration.

Meza-Buendia 2022 PLoS One2022Meza-Buendia AK, Aparicio-Trejo OE, Díaz F, Caamal-Monsreal C, Pedraza-Chaverri J, Álvarez-Delgado C, Paschke K, Rosas C (2022) High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart. https://doi.org/10.1371/journal.pone.0273554
MiPNet02.05 DatLab2 O2Kinetics1997-01
O2k-Manual

DatLab 2. Analysis of oxygen kinetics.

MiPNet03.03 Internat-Oxygraph-Workshops1991-
O2k-Workshops.png

Innsbruck AT, since 1991. International oxygraph workshops on high-resolution respirometry, IOC.

MiPNet04.05 Titration-Injection1999-01
O2k-Protocols contents

From step titration to ramp injection: Uncoupling by FCCP with TIP.

MiPNet06.01 O2k-Overview2001
Oroboros

Gnaiger E (2001) The Oxygraph for high-resolution respirometry. Mitochondr Physiol Network 06.01.

MiPNet06.03 POS-calibration-SOP2023-02-06
O2k-Manual

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

MiPNet06.05 Test Experiments on O2k-Specifications2010
Logo OROBOROS INSTRUMENTS.jpg

Gnaiger E (2010) Test experiments on specifications of the Oroboros Oxygraph. Mitochondr Physiol Network 6.5.

MiPNet06.06 Chemical O2 background2015-04-27
O2k-Manual

Oxygraph assay of cytochrome c oxidase activity: chemical background correction.

MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents

High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.

MiPNet08.12 IOC222011-12-11
O2k-Protocols

NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.

MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols

An experiment with high-resolution respirometry: coupling control in cell respiration.

MiPNet12.10 TIP2k-manual2021-07-08
O2k-Manual

Titration-Injection microPump TIP2k manual.

MiPNet12.11 MitoRespiration2014-02-20
O2k-Protocols

Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.

MiPNet14.06 Instrumental O2 background2023-10-19
O2k-Manual

O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.

MiPNet15.03 O2k-MultiSensor-ISE2021-08-02
O2k-Manual

O2k-MultiSensor system with ion selective electrodes (ISE).

MiPNet15.05 NO-manual2016-04-21
O2k-Manual

O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.

MiPNet17.03 Shredder vs Fibers2015-06-18
O2k-Protocols

Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.

MiPNet17.04 CitrateSynthase2020-04-18
O2k-Protocols

Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.

MiPNet17.05 O2k-Fluo LED2-Module2021-06-22
O2k-Manual

O2k-Fluo LED2-Module.

MiPNet18.10 O2k-Specifications2024-03-26
Oroboros

O2k-specifications for respirometry and comprehensive OXPHOS analysis.

MiPNet19.01 O2k-Core Manual Series F DatLab 52014-03
O2k-Manual

O2k-Core Manual - up to O2k-Series F and DatLab 5.

MiPNet19.03 O2k-cleaning and ISS2023-03-14
O2k-Manual

O2k-Chamber cleaning SOP and Integrated Suction System (ISS).

MiPNet19.14 SOP Hamilton microsyringes2023-09-28
O2k-Manual

SOP for manual O2k-titrations with Hamilton microsyringes.

MiPNet19.18 O2k-Series G Core manual2016-08-08
O2k-Manual

O2k-Core manual contents.

MiPNet19.18A O2k-Series G: Start2016-08-08
O2k-Manual

Oxygraph-2k: start high-resolution respirometry.

MiPNet19.18B POS-service2021-06-23
O2k-Manual

Service of the polarographic oxygen sensor OroboPOS.

MiPNet19.18C DatLab 6: Guide2016-08-24
O2k-Manual

DatLab-guide through the menus.

MiPNet19.18D O2k-Series G and DatLab 6: Calibration2016-08-24
O2k-Protocols

O2k-calibration by DatLab 6.

MiPNet19.18E O2 flux analysis - DatLab 62016-08-08
O2k-Manual

Oxygen flux analysis: DatLab real-time.

MiPNet20.04 O2k-checklist2021-03-12
O2k-Manual

O2k-checklist: get started with an O2k-experiment.

MiPNet20.13 Safranin mt-membranepotential2019-06-24
O2k-Protocols

O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.

MiPNet20.14 AmplexRed H2O2-production2019-06-24
O2k-Protocols

O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.

MiPNet20.17 MiP2015 Lucni Bouda2015
MiPsociety

Pecina P et al, eds (2015) Mitochondrial physiology conference. MiP2015. Mitochondr Physiol Network 20.17:87 pp.

MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols

SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.

MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols

MitoFit DatLab proficiency test.

MiPNet21.12 Comparison mt respiration media2016-10-18
O2k-Protocols

O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.

MiPNet21.13 Comparison mt-preparations2016-10-18
O2k-Protocols

O2k-Protocols: Mitochondrial preparations for HRR.

MiPNet21.14 Reference sample HRR2016-10-19
O2k-Protocols

Development of a reference sample for HRR.

MiPNet21.16 DatLab 7 Innovations2019-09-23
About Oroboros

DatLab 7: innovations.

MiPNet21.17 BloodCellsIsolation2020-10-22
O2k-Protocols

O2k-Protocols: Isolation of blood cells for HRR.

MiPNet22.11 O2k-FluoRespirometer manual2023-03-14
O2k-Manual

O2k-FluoRespirometer manual.

MiPNet22.16 DatLab 7 Innovations: DL-Protocols2019-09-13
O2k-Protocols

DatLab 7.4 Innovations: DL-Protocols

MiPNet23.12 DL-Protocols help2019-09-13
O2k-Protocols

DatLab Protocols: Help DatLab 7.

MiPNet23.15 O2k-pH ISE-Module2018-09-08
O2k-Manual

Measurement of proton flux with the O2k-pH ISE-Module.

MiPNet24.06 Oxygen flux analysis - DatLab 7.42020-04-16
O2k-Protocols

Oxygen flux analysis with DatLab 7.4.

MiPNet24.08 Safranin Analysis Template2020-05-13
O2k-Protocols

Excel template for safranin data analysis.

MiPNet24.09 Data analysis of mt-membrane potential2021-09-16
O2k-Protocols

Data analysis of mt-membrane potential.

MiPNet24.10 H2O2 flux analysis2021-10-22
O2k-Protocols

Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4

MiPNet24.11 mtMP calculation2019-08-05
O2k-Protocols

Mitochondrial membrane potential calculation

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