Description
Octanoyl carnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine.
Abbreviation: Oct
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Substrate and metabolite
Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:
- octanoyl-CoA + L-carnitine β CoA + L-octanoylcarnitine
Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
Application in HRR
Oct: Octanoyl carnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85. Alternative: (Β±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 100 mM stock solution (dissolved in H2O):
- 1) weigh 32.4 mg of octanoyl carnitine and dissolve in 1 ml H2O
- 2) divide into 0.2 ml portions
- 3) store frozen at -20 Β°C.