Rasmussen 2003 Eur J Physiol
Rasmussen UF, Krustrup P, Kjaer M, Rasmussen HN (2003) Human skeletal muscle mitochondrial metabolism in youth and senescence: no signs of functional changes in ATP formation and mitochondrial oxidative capacity. Pflugers Arch – Eur J Physiol 446:270-78. |
Rasmussen UF, Krustrup P, Kjaer M, Rasmussen HN (2003) Eur J Physiol
Abstract: The mitochondrial theory of ageing was tested. Isolated mitochondria from the quadriceps muscle from normal, healthy, young (age 20+ years, n=12) and elderly (70+ years, n=11) humans were studied in respiratory experiments and the data expressed as activities of the muscle. In each group, the subjects exhibited a variation of physical activity but, on average, the groups were representative for their age with maximum O(2) consumption rate of 50+/-9 and 34+/-13 ml min(-1) kg(-1) (mean+/-SD), respectively. Thirteen different activities were assayed. alpha-Glycerophosphate oxidation was lower in the 70+ group (38%, P~0.001), as was the respiratory capacity for fatty acids (19%, P~0.03). The remaining eleven activities, including those of the central bioenergetic reactions, were not lower in the 70+ group. Pyruvate and alpha-ketoglutarate dehydrogenase activities (i.e. the tricarboxylic acid cycle turnover) and the respiratory chain activity could all account for ~14 mmol O(2) min(-1) kg(-1) muscle (37 degrees C). The capacity for aerobic ATP synthesis was ~35 mmol ATP min(-1) kg(-1). The mitochondrial capacities were far in excess of whole-body performance. They were related to physical activity, but not to age. The mitochondrial theory of ageing, which attributes the age-related decline of muscle performance to decreased mitochondrial function, is incompatible with these results. • Keywords: Age effects, Ageing, Human skeletal muscle, Isolated mitochondria, Oxidative phosphorylation, Oxygen uptake, Quadriceps muscle, Respiration • Bioblast editor: Gnaiger E
MitoEAGLE VO2max/BME data base
- Human vastus lateralis
- 12 males
- 24 years
- Range of differenct endurance activities
- H = 1.79 m
- M = 75 kg
- BME = 1.12
- BMI = 23.4 kg·m-2
- VO2max/BM = 50.0 mL·min-1·kg-1
- Isolated mitochondria; 25 °C; GSP; conversions: Gnaiger 2009 Int J Biochem Cell Biol
- JO2,P(NS) = 120.6 µmol·s-1·kg-1 wet muscle mass (37 °C)
- 10.3 µM mt-protein/mg mw
- Human vastus lateralis
- 1 female & 10 males
- 72 years
- Range of differenct endurance activities
- H = 1.75 m
- M = 80 kg
- BME = 1.28
- BMI = 26.1 kg·m-2
- VO2max/BM = 34.0 mL·min-1·kg-1
- Isolated mitochondria; 25 °C; GSP; conversions: Gnaiger 2009 Int J Biochem Cell Biol
- JO2,P(NS) = 115.1 µmol·s-1·kg-1 wet muscle mass (37 °C)
- 10.3 µM mt-protein/mg mw
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Labels: MiParea: Exercise physiology;nutrition;life style
Pathology: Aging;senescence
Organism: Human Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex V;ATP synthase Regulation: Substrate
Pathway: N, NS
MitoEAGLE BME, BMI, VO2max, BME