Honzik 2006 Placenta: Difference between revisions
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{{Publication | {{Publication | ||
|title=Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Specific properties of heavy fraction of mitochondria from human-term placenta - Glycerophosphate-dependent hydrogen peroxide production. Placenta 2006 27: 348- | |title=Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Specific properties of heavy fraction of mitochondria from human-term placenta - Glycerophosphate-dependent hydrogen peroxide production. Placenta 2006 27:348-56. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/15949844 PMID: 15949844] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/15949844 PMID: 15949844] | ||
|authors=Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J | |authors=Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J | ||
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{{Labeling | {{Labeling | ||
|organism=Human | |organism=Human | ||
|preparations=Intact | |preparations=Intact organ, Isolated mitochondria | ||
|enzymes=Complex IV; | |enzymes=Complex IV;cytochrome c oxidase, TCA cycle and matrix dehydrogenases | ||
|injuries=RONS | |injuries=Oxidative stress;RONS, Mitochondrial disease | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Spectrophotometry; Spectrofluorimetry | |additional=Spectrophotometry; Spectrofluorimetry | ||
}} | }} |
Revision as of 11:12, 13 February 2015
Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Specific properties of heavy fraction of mitochondria from human-term placenta - Glycerophosphate-dependent hydrogen peroxide production. Placenta 2006 27:348-56. |
Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Placenta
Abstract: Mitochondrial respiratory chain enzyme Complexes are present in placenta at proportion similar to other tissues with exception of glycerophosphate dehydrogenase (mGPDH) which is expressed at a very high rate. As shown by Western blot quantification and respiratory chain enzyme activity measurements, the specific content of mGPDH is similar to that of succinate dehydrogenase or NADH dehydrogenase. Using fluorometric probe dichlorodihydrofluorescein diacetate we found that placental mitochondria display high rate of glycerophosphate-dependent hydrogen peroxide production. This was confirmed by oxygraphic detection of glycerophosphate-induced, KCN- or antimycin A-insensitive oxygen uptake. Hydrogen peroxide production by mGPDH was highly activated by one-electron acceptor, potassium ferricyanide and it was depressed by inhibitors of mGPDH and by cytochrome c. Our results indicate that mGPDH should be considered as an additional source of reactive oxygen species participating in induction of oxidative stress in placenta. โข Keywords: Human placenta, Mitochondria, Glycerophosphate dehydrogenase, ROS
โข O2k-Network Lab: CZ_Prague_Zeman J, CZ Hradec Kralove Cervinkova Z
Labels:
Stress:Oxidative stress;RONS, Mitochondrial disease Organism: Human
Preparation: Intact organ, Isolated mitochondria Enzyme: Complex IV;cytochrome c oxidase, TCA cycle and matrix dehydrogenases
Coupling state: OXPHOS
HRR: Oxygraph-2k
Spectrophotometry; Spectrofluorimetry