Klingenberg 1982 Eur J Biochem: Difference between revisions
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{{Publication | {{Publication | ||
|title=Klingenberg M, Grebe K, Appel M (1982) Temperature dependence of ADP/ATP translocation in mitochondria. Eur J Biochem 126: 263- | |title=Klingenberg M, Grebe K, Appel M (1982) Temperature dependence of ADP/ATP translocation in mitochondria. Eur J Biochem 126:263-69. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/6290218 PMID: 6290218] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/6290218 PMID: 6290218] | ||
|authors=Klingenberg M, Grebe K, Appel M | |authors=Klingenberg M, Grebe K, Appel M | ||
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|organism=Rat, Bovines | |organism=Rat, Bovines | ||
|tissues=Heart, Liver | |tissues=Heart, Liver | ||
|preparations=Isolated | |preparations=Isolated mitochondria, SMP | ||
|enzymes=Adenine | |enzymes=Adenine nucleotide translocase | ||
|topics=Temperature | |topics=Temperature | ||
|additional=Beef | |additional=Beef | ||
}} | }} | ||
Latest revision as of 16:47, 11 February 2015
| Klingenberg M, Grebe K, Appel M (1982) Temperature dependence of ADP/ATP translocation in mitochondria. Eur J Biochem 126:263-69. |
Klingenberg M, Grebe K, Appel M (1982) Eur J Biochem
Abstract: The temperature dependence of the adenine nucleotide exchange in mitochondria has been determined by employing a rapid mixing, quenching and sampling apparatus and the inhibitor quench-back exchange method. Thus the exchange is resolved down to 0.1 s. Rates are evaluated from accumulating the time-dependent progress at about 10 points. The exchange rate in liver mitochondria was determined from -10 degrees C to + 10 degrees C in the presence of 20% glycol, from 0 degrees C to 25 degrees C, and from 20 degrees C to 40 degrees C under partial inhibition by carboxyatractylate. The total range between -10 degrees C to + 40 degrees C has only one temperature break at 13 degrees C. From the Arrhenius plot between -10 degrees C to + 13 degrees C, EA approximately equal to 140 kJ and above 13 degrees C, EA approximately equal to 56 kJ is evaluated, corresponding to a Q10 of 8 and 2 respectively. In beef heart mitochondria the exchange rate was measured between 0 degrees C and 20 degrees C, and between 15 degrees C and 30 degrees C under partial inhibition with carboxyatractylate. There is a temperature break around 14 degrees C with EA approximately equal to 143 kJ between 0 degrees C and 14 degrees C and EA approximately equal to 60 kJ from 15 degrees C to 30 degrees C. The extrapolated translocation rates at 37 degrees C are 500 and 1800 mumol min-1 (g protein)-1 for rat liver and for beef heart mitochondria respectively. The temperature break is suggested to reflect a conformation change since there is no reversed break at low temperature, the temperature break changes in sonic particles and no lipid phase transition at 14 degrees C in mitochondria has been reported.
Labels:
Organism: Rat, Bovines
Tissue;cell: Heart, Liver
Preparation: Isolated mitochondria, SMP
Enzyme: Adenine nucleotide translocase
Regulation: Temperature
Beef
