Octanoylcarnitine: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 28: | Line 28: | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 ยตL using a 10 ยตL Hamilton syringe | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 ยตL using a 10 ยตL Hamilton syringe | ||
::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05. | ::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05. | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | }} |
Revision as of 10:52, 9 November 2020
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
Application in HRR
- Oct: Octanoylcarnitine ((ยฑ)-Octanoylcarnitine chloride, C15H30NO4Cl); APExBIO Technology, Cat. No. B6371, 50 mg, store at -20ยฐC; FW = 323.86.
- Alternative: (ยฑ)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
- TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
- General tips: For obtaining a higher solubility, warm the tube at 37 โ and shake it in the ultrasonic bath for a while.
- Alternative: (ยฑ)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 ยตL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 ยฐC.
- ยป O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 ยตL using a 25 ยตL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 ยตL using a 10 ยตL Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
MitoPedia topics: Substrate and metabolite