Chinopoulos 2009: Difference between revisions
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|mipnetlab=HU Budapest Chinopoulos C | |mipnetlab=HU Budapest Chinopoulos C | ||
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|organism=Rat | |||
|tissues=Heart, Nervous system, Liver | |||
|enzymes=Adenine nucleotide translocase | |enzymes=Adenine nucleotide translocase | ||
|topics=ADP, ATP, ATP production | |topics=ADP, ATP, ATP production | ||
|additional=MgG, Safranin, ANT, | |additional=MgG, Safranin, ANT, | ||
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::::ยป Fluorometry protocol for [[Magnesium green]] |
Revision as of 13:00, 5 March 2020
Chinopoulos Christos, Vajda Szilvia, Csanady Laszlo, Mandi Miklos, Mathe Katalin, Adam-Vizi Vera (2009) A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT. Biophys J 96, 2490-504. |
Chinopoulos Christos, Vajda Szilvia, Csanady Laszlo, Mandi Miklos, Mathe Katalin, Adam-Vizi Vera (2009) Biophys J
Abstract: A novel method exploiting the differential affinity of ADP and ATP to Mg(2+) was developed to measure mitochondrial ADP-ATP exchange rate. The rate of ATP appearing in the medium after addition of ADP to energized mitochondria, is calculated from the measured rate of change in free extramitochondrial [Mg2+] reported by the membrane-impermeable 5K+ salt of the Mg2+-sensitive fluorescent indicator, Magnesium Green, using standard binding equations. The assay is designed such that the adenine nucleotide translocase (ANT) is the sole mediator of changes in [Mg2+] in the extramitochondrial volume, as a result of ADP-ATP exchange. We also provide data on the dependence of ATP efflux rate within the 6.8-7.8 matrix pH range as a function of membrane potential. Finally, by comparing the ATP-ADP steady-state exchange rate to the amount of the ANT in rat brain synaptic, brain nonsynaptic, heart and liver mitochondria, we provide molecular turnover numbers for the known ANT isotypes.
โข Keywords: Adenine nucleotide translocase, ANT, ATP-ADP exchange, Magnesium Green
โข Bioblast editor: Cardoso Luiza HD,
โข O2k-Network Lab: HU Budapest Chinopoulos C
Labels:
Organism: Rat
Tissue;cell: Heart, Nervous system, Liver
Enzyme: Adenine nucleotide translocase Regulation: ADP, ATP, ATP production
MgG, Safranin, ANT
- ยป Fluorometry protocol for Magnesium green