P-L net OXPHOS capacity: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr='' | |abbr=''P-L'' | ||
|description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', '' | |description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', ''P-L'', is the [[OXPHOS capacity]] corrected for [[LEAK respiration]], ''P-L''. ''P-L'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ''โP'', strictly coupled to phosphorylation, ''Pยป'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''P-L''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]] ''L'' as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher ''pmF''. | ||
|info=[[Gnaiger 2020 MitoPathways]] | |info=[[Gnaiger 2020 MitoPathways]] | ||
}} | }} | ||
Communicated by [[Gnaiger E]] (2014-08-09) last update 2020-11-07. | |||
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2020 MitoPathways |The Blue Book 2020]]]] | |||
Communicated by [[Gnaiger E]] 2014-08-09 | |||
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger | |||
== Coupling control states for '' | == Coupling control states for ''P-L'' == | ||
::ยป [[Respiratory state]] | ::ยป [[Respiratory state]] | ||
* [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS capacity|OXPHOS capacity]] [[OXPHOS capacity]], ''P'' = ''Pยด''-ROX | * [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS capacity|OXPHOS capacity]] [[OXPHOS capacity]], ''P'' = ''Pยด''-ROX | ||
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== Flux control | == Flux control efficiency == | ||
::ยป [[Flux control | ::ยป [[Flux control efficiency]] | ||
* [[Coupling control | * [[Coupling control efficiency]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--P.jpg|50 px|link=OXPHOS-coupling efficiency |OXPHOS-coupling efficiency]] ''P-L'' coupling control efficiency, [[OXPHOS-coupling efficiency]]: ''j<sub>P-L</sub>'' = (''P-L'')/''P'' = 1-''L/P'' | ||
== Compare == | == Compare == | ||
:: [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] [[Free ET capacity]], | :: [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] [[Free ET capacity]], ''E-L'' | ||
:: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], | :: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], (''P-L'')/''E'' | ||
:: [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] [[ET-coupling efficiency]], ''E-L'' control | :: [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] [[ET-coupling efficiency]], ''E-L'' control efficiency: ''j<sub>E-L</sub>'' = (''E-L'')/''E'' = 1-''L/E'' | ||
* Intact cells: [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''โR'' = ''R-L'' | * Intact cells: [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''โR'' = ''R-L'' |
Revision as of 19:12, 7 November 2020
Description
The free OXPHOS capacity, P-L, is the OXPHOS capacity corrected for LEAK respiration, P-L. P-L is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, โP, strictly coupled to phosphorylation, Pยป, and nonphosphorylating LEAK respiration, LP, compensating for proton leaks, slip and cation cycling: P = P-L+LP. It is frequently assumed that LEAK respiration L as measured in the LEAK state, overestimates the LEAK component of respiration, LP, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LP. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher pmF.
Abbreviation: P-L
Reference: Gnaiger 2020 MitoPathways
Communicated by Gnaiger E (2014-08-09) last update 2020-11-07.
Coupling control states for P-L
- Reference state, ZX: OXPHOS capacity, P = Pยด-ROX
- Background state, YX: LEAK respiration, L = Lยด-ROX
- Metabolic control variable, X=ZX-YX: Scope of ADP stimulation, free OXPHOS capacity, โP = P-L
Flux control efficiency
- Coupling control efficiency, 1-YX/ZX: P-L coupling control efficiency, OXPHOS-coupling efficiency: jP-L = (P-L)/P = 1-L/P
Compare
- Free ET capacity, E-L
- netOXPHOS control ratio, (P-L)/E
- ET-coupling efficiency, E-L control efficiency: jE-L = (E-L)/E = 1-L/E
- Intact cells: Free ROUTINE activity, โR = R-L