Cyclosporin A: Difference between revisions
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== Inhibition of PTP by CsA == | == Inhibition of PTP by CsA == | ||
:::* Gainutdinov T, Molkentin JD, Siemen D, Ziemer M, Debska-Vielhaber G, Vielhaber S, Gizatullina Z, Orynbayeva Z, Gellerich FN (2015) Knockout of cyclophilin D in Ppif<sup>-</sup>/<sup>-</sup> mice increases stability of brain mitochondria against Ca<sup>2+</sup> stress. Arch Biochm Biophys 579:40β46. | :::* Gainutdinov T, Molkentin JD, Siemen D, Ziemer M, Debska-Vielhaber G, Vielhaber S, Gizatullina Z, Orynbayeva Z, Gellerich FN (2015) Knockout of cyclophilin D in Ppif<sup>-</sup>/<sup>-</sup> mice increases stability of brain mitochondria against Ca<sup>2+</sup> stress. Arch Biochm Biophys 579:40β46. | ||
:::* Briston T, Roberts M, Lewis S, Powney B, Staddon JM, Szabadkai G, Duchen MR (2017) Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability.Β Scientific reportsΒ 7: 10492. | |||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
{{MitoPedia O2k and high-resolution respirometry}} | {{MitoPedia O2k and high-resolution respirometry}} |
Revision as of 09:20, 22 May 2019
Description
Cyclosporin A (CsA) is a cyclic undecapeptide from an extract of soil fungi that binds the cyclophilin D and thus preventing the formation of the mitochondrial permeability transition pore. The interaction of CsA with the cyclophilin D is phosphate mediated but the full mechanism of interaction is not well understood. For example, the deficiency of cyclophilin D in KO models does not prevent mitochondria from permeability transition and from CsA inhibition. Moreover, it is also a is a calcineurin inhibitor and potent immunosuppressive agent used largely as a means of prophylaxis against cellular rejection after solid organ transplantation.
Abbreviation: CsA
MitoPedia topics:
Inhibitor
Application in HRR
- CsA:Cyclosporin A (C62H111N11O12); Sigma 30024, 25 mg, store commercial stock at 2-8Β°C; FW = 1202.61 gr/mol (β₯ 95%).
- Preparation of 400 Β΅M stock solution (dissolved in 99.9% ethanol)
- Weigh 1 mg of CsA and dissolve in 2.11 ml of ethanol absolute (99.9%).
- Divide in aliquots.
- Store frozen at -20 Β°C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 Β΅l using a 10 Β΅l syringe (2 ml O2k-chamber).
- Final concentration: 1 Β΅M.
Even if 1 Β΅M is recommended as the working concentration in most of the publications it is highly relevant to test the optimal concentration for your research model.
Inhibition of PTP by CsA
- Gainutdinov T, Molkentin JD, Siemen D, Ziemer M, Debska-Vielhaber G, Vielhaber S, Gizatullina Z, Orynbayeva Z, Gellerich FN (2015) Knockout of cyclophilin D in Ppif-/- mice increases stability of brain mitochondria against Ca2+ stress. Arch Biochm Biophys 579:40β46.
- Briston T, Roberts M, Lewis S, Powney B, Staddon JM, Szabadkai G, Duchen MR (2017) Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability. Scientific reports 7: 10492.