Talk:Bioblast: Difference between revisions
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*Term of the week: '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - a hallmark of high-resolution respirometry is a matching '''high-resolution terminology'''. | *Term of the week: '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - a hallmark of high-resolution respirometry is a matching '''high-resolution terminology'''. | ||
::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''. | ::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''. | ||
====Bioblast alert ## to select==== | |||
*O2k-Publication: '''Protocols for HRR: from intact cells to permeabilized muscle fibres''' | |||
::[[Pesta 2012 MethodsMolBiol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol. Biol. 810: 25-58.]] | |||
====Bioblast alert ## to select==== | ====Bioblast alert ## to select==== |
Revision as of 12:22, 14 November 2011
Bioblast alert
Bioblast alert - in preparation
Bioblast alert #02
- O2k-Publication: Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart β a paradox resolved by a dyscoupling mechanism.
- Scientific service: Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
- Term of the week: Uncoupled, non-coupled, dyscoupled - a hallmark of high-resolution respirometry is a matching high-resolution terminology.
- Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.
Bioblast alert ## to select
- O2k-Publication: Protocols for HRR: from intact cells to permeabilized muscle fibres
Bioblast alert ## to select
- O2k-Publication: Fluorescence in the O2k
- Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print]
- Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am. J. Physiol. Heart Circ. Physiol. 300: H118-124.
- MiPNet Reference Laboratory: NZ Auckland Hickey AJ - 6 O2k, guest lecturer at IOC66, joint project (Marsden), guest tutor at IOC59.
Bioblast alert ## to select
- O2k-Publication: Efficiency, basal respiration, and oxygen kinetics in human muscle or Mitochondrial efficiency and P/O flux ratios: new perspectives in mitochondrial physiology (see Journal club);
- Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 13: 149-159.
- Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J. 25: 2843-2852.
- Before alert, ask to join as MiPNet Reference Lab, and present new MiPNet Reference Lab
- Term: efficiency versus P/O ratio
Bioblast alert - previous
Bioblast alert #01 (2011-11-11)
- O2k-Fluorimetry Workshop IOC66: 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the O2k-Fluorescence Module will be delivered in April 2012, for orders received by end of January 2012.
- O2k-Publication: A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
- Scientific service: ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
- Term of the month: Basal respiration - a hallmark of high-resolution respirometry is a matching high-resolution terminology.
Bioblast accessed
- The Bioblast Wiki has been accessed >20,000 times (2011-10-05)