P-L net OXPHOS capacity: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''โP'' | |abbr=''โP'' | ||
|description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', ''โP'', is the [[OXPHOS capacity]] corrected for [[LEAK respiration]], ''โP'' = ''P-L''. ''โP'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ''โP'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''โP''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher ฮ''p''<sub>mt</sub>. | |description=[[Image:P-L.jpg|50 px|Free OXPHOS-capacity]] The '''free OXPHOS-capacity''', ''โP'', is the [[OXPHOS-capacity]] corrected for [[LEAK respiration]], ''โP'' = ''P-L''. ''โP'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS-capacity, ''โP'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''โP''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher ฮ''p''<sub>mt</sub>. | ||
|info=[[Gnaiger 2014 MitoPathways]] | |info=[[Gnaiger 2014 MitoPathways]] | ||
}} | }} | ||
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== Coupling control states for ''โP'' == | == Coupling control states for ''โP'' == | ||
::ยป [[Respiratory state]] | ::ยป [[Respiratory state]] | ||
* [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[OXPHOS capacity]], ''P'' = ''Pยด''-ROX | * [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS]] [[OXPHOS-capacity]], ''P'' = ''Pยด''-ROX | ||
* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]], ''L'' = ''Lยด''-ROX | * [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK-respiration|LEAK]] [[LEAK-respiration]], ''L'' = ''Lยด''-ROX | ||
* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] Scope of ADP stimulation, [[free OXPHOS capacity]], ''โP'' = ''P-L'' | * [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:P-L.jpg|50 px|link=Free OXPHOS-capacity |Free OXPHOS-capacity]] Scope of ADP stimulation, [[free OXPHOS-capacity]], ''โP'' = ''P-L'' | ||
Revision as of 16:25, 26 November 2018
Description
The free OXPHOS-capacity, โP, is the OXPHOS-capacity corrected for LEAK respiration, โP = P-L. โP is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS-capacity, โP, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, LP, compensating for proton leaks, slip and cation cycling: P = โP+LP. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, LP, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LP. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher ฮpmt.
Abbreviation: โP
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
Respiratory state,
Recommended
MitoPedia topics:
EAGLE
Communicated by Gnaiger E 2014-08-09, edited 2015-01-19.
Coupling control states for โP
- Reference state, ZX: OXPHOS-capacity, P = Pยด-ROX
- Background state, YX: LEAK-respiration, L = Lยด-ROX
- Metabolic control variable, X=ZX-YX: Scope of ADP stimulation, free OXPHOS-capacity, โP = P-L
Flux control factor
- ยป Flux control factor, FCF
- Coupling control factor, 1-YX/ZX: P-L coupling control factor, OXPHOS coupling efficiency: jโP = โP/P =(P-L)/P = 1-L/P
Compare
- Free ET-capacity, โE = E-L
- netOXPHOS control ratio, โP/E control ratio: โP/E = (P-L)/E
- ET-coupling efficiency, E-L control factor: jโE = โE/E = (E-L)/E = 1-L/E
- Intact cells: Free ROUTINE activity, โR = R-L