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Talk:SUIT-006 MgG mt D055

From Bioblast

Chemicals and syringes

Step Chemical(s) and link(s) Comments
MgG Magnesium Green
Mg MgCl2
Step Chemical(s) and link(s) Comments
1PM Pyruvate (P) and Malate (M) Can be substituted by other combination of substrates
2D ADP (D) preparation for MgG assay For this protocol, the ADP must be prepared without Mg2+ salts.
3Cat Carboxyatractyloside (Cat) Can be substituted for oligomycin
4U SF6847 Can be substituted for other uncoupler
5Ama Antimycin A (Ama) Can be substituted for other CIII or CIV inhibitor
Suggested stock concentrations are shown in the specific DL-Protocol.