Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Talk:Permeabilization of plasma membrane

From Bioblast

high-resolution terminology - matching measurements at high-resolution

Talk:Permeabilization of plasma membrane


Selective permeabilization of plasma membrane or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (e.g., ADP, cytochrome c) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (e.g., liver tissue) and chemically by mild detergents (i.e., digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue, Permeabilized tissue or cells.

ยป MitoPedia topic: Mitochondrial preparations

Reference: Doerrier 2018 Methods Mol Biol

MitoPedia methods: Respirometry 

MitoPedia topics: Sample preparation 

MitoPedia: permeabilization of plasma membrane


Bioblast linkReferenceYear
Gnaiger E et al โ€• MitoEAGLE Task Group (2020) Mitochondrial physiology. 2020.1


Preparation: Permeabilized tissue, Permeabilized cells, Permeabilized muscle fibers