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Talk:Permeabilization of plasma membrane

From Bioblast


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Talk:Permeabilization of plasma membrane

Description

Selective permeabilization of plasma membrane or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (e.g., ADP, cytochrome c) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (e.g., liver tissue) and chemically by mild detergents (i.e., digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue, Permeabilized tissue or cells.

ยป MitoPedia topic: Mitochondrial preparations


Reference: Doerrier 2018 Methods Mol Biol



MitoPedia methods: Respirometry 



MitoPedia topics: Sample preparation 



MitoPedia: permeabilization of plasma membrane


References

Bioblast linkReferenceYear
Gnaiger E et al โ€• MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1BEC 2020.1


Labels:



Preparation: Permeabilized tissue, Permeabilized cells, Permeabilized muscle fibers