Ost 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
|Ost 2015 Fatty Acid Oxidation O2k-Network Discussion Forum|
Ost M (2015)
Right now we´re using octanoyl carnitine (OC) when examining mouse skeletal muscle permeabilized fiber respiration. We ususally start with Malate (2mM) and OC (0.2mM), then add ADP (5mM) in order to assess fatty acid oxidative capacity. For Soleus muscle fibers we got an average respiration of 60 pmol/(s*mg fiber ww).
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Octanoylcarnitine, Malate, ADP