MiPNet03.02 Chemicals-Media

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MiPNet03.02 Chemicals-Media

Publications in the MiPMap
Selected media and chemicals for respirometry with mitochondrial preparations.

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O2k-Protocols

Oroboros (2016-08-30) Mitochondr Physiol Network

Abstract: Fontana-Ayoub M, Fasching M, Gnaiger E (2016) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(18):1-10. Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of results obtained in different laboratories. Efforts towards standardization are important for the advancement of mitochondrial physiology.

» Product: Oroboros O2k, O2k-Catalogue


O2k-Network Lab: AT Innsbruck Oroboros

Supplementary information

» MiPNet09.12 O2k-Titrations; MitoPedia
» Cytochrome c control factor
» For calculations, see Excel file: O2k-Titrations.xls
» For pH adjustment of BIOPS solution, note the temperature dependence:
  • at 0 °C pH = 7.1
  • at 23 °C pH = 6.75

Cited by

  • Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
  • Komlodi et al (2022) Hydrogen peroxide production, mitochondrial membrane potential and the coenzyme Q redox state measured at tissue normoxia and experimental hyperoxia in heart mitochondria. MitoFit Preprints 2021 (in prep)


Labels: MiParea: Instruments;methods 





HRR: O2k-Protocol 

O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH