Jacobs 2013 J Gerontol A Biol Sci Med Sci

From Bioblast
Jump to navigation Jump to search
Publications in the MiPMap
Jacobs R, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function. J Gerontol A Biol Sci Med Sci 68:1010-22.

» PMID: 23371970

Jacobs R, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) J Gerontol A Biol Sci Med Sci

Abstract: The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

Keywords: Senescence, Slow-twitch oxidative muscle, Type 2 fast-twitch glycolytic muscle, Function theory of aging.; Mitochondria; Respiratory chain

O2k-Network Lab: CH Zurich Lundby C, CH Zurich Gassmann M, US CO Colorado Springs Jacobs RA


Labels: MiParea: Respiration  Pathology: Aging;senescence 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex III 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX  HRR: Oxygraph-2k