ISE-Ca2+ Membranes

ISE-Ca2+ Membranes

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.

To be used with the O2k-TPP+ ISE-Module.

Reference: Product details and purchase information

Technical support

The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al^[1]. The limit of detection is less than 10 nM in Ca²⁺ buffered calibration solutions. The main difficulty in establishing reliable Ca²⁺ measurements is not the Ca²⁺ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions.

Additional resources

» ISE Manual

» O2k-TPP+ ISE-Module

References

↑ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca²⁺-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry

[1] Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca²⁺-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.

[1]