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ISE-Ca2+ Membranes

From Bioblast


                  


O2k-Open Support

ISE-Ca2+ Membranes



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O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

Ca2+ membranes.jpg

ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.

To be used with the O2k-TPP+ ISE-Module.


Reference: Product details and purchase information


Technical support

The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al[1]. The limit of detection is less than 10 nM in Ca2+ buffered calibration solutions. The main difficulty in establishing reliable Ca2+ measurements is not the Ca2+ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions.

Additional resources

ยป ISE Manual
ยป O2k-TPP+ ISE-Module

References

  1. โ†‘ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca2+-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.


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