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Talk:Mitochondrial Diagnostics
Mitochondrial Diagnostics
Mitochondrial Diagnostics
- Duration: 4 years
- Start: 2022-04-01 to 2026-03-31
About Mitochondrial Diagnostics
- Mitochondrial Diagnostics builds upon the concept of standardized functional analyses and interpretation of bioenergetic snapshots of (1) cryopreserved PBMCs obtained from minimally invasive blood samples, (2) established cell cultures subjected to pharmacological screening, and (3) mitochondrial preparations obtained from animal or human tissue samples [3,4].
Aims
- Mitochondrial Diagnostics aims at establishing laboratories for mitochondrial respiratory diagnostics offering bioenergetic snapshots in collaboration with academic and clinical partners, interest groups, funding agencies, and investors.
- Bioenergetic snapshots are obtained from substrate-uncoupler-inhibitor titration protocols in respirometric assays interrogating specific mitochondrial functions [4]. Depending on the diagnostic focus, optimized mitochondrial preparations are applied covering the entire spectrum from living or permeabilized cells to mitochondria isolated from cultured cells or tissues.
References
- Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. PLoS ONE 17:e0264496.
- Ioannidis JPA, Greenland S, Hlatky MA, Khoury MJ, Macleod MR, Moher D, Schulz KF, Tibshirani R (2014) Increasing value and reducing waste in research design, conduct, and analysis. Lancet 383:166-75.
- Gnaiger E (2021) Bioenergetic cluster analysis β mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Coordinator
- Verena Laner - Oroboros project management
Network
Participants
- VASCage GmbH Research Centre on Vascular Ageing and Stroke - www.vascage.at
- Oroboros Instruments GmbH - www.oroboros.at
- Erich Gnaiger, Ph.D. - PI
- Sabine Schmitt, Ph.D
- Carolina Gnaiger - Oroboros project management
- Verena Laner - Oroboros project management
Oroboros project involvement
Mitochondrial Diagnostics Events
When | Where | |
---|---|---|
Club17 2024 Innsbruck AT | 2024-03-21 | Innsbruck AT, 2024 Mar 21. Club17 |
Life Sciences Partnering 2023 Innsbruck AT | 2023-10-19 | Innsbruck, AT, Oct 19, 2023. Life Sciences Partnering 2023 |
2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT | 2023-10-16 | Innsbruck AT, 2023 Oct 16. 2nd Workshop on Mitochondrial Functional Diagnostics |
1st Workshop on Mitochondrial Functional Diagnostics Innsbruck AT | 2023-07-10 | Innsbruck AT, 2023 Jul 10. 1st Workshop on Mitochondrial Functional Diagnostics |
Mitochondrial Diagnostics Publications
Links
News and articles
- 18 Millionen fΓΌr Schlaganfallforschung - tirol.orf.at, online 2022-02-01
Support
- This project has received funding from the Austrian Research Promotion Funding Agency (FFG).