MiPschool 2007 Schroecken AT Programme
MiPsummer Programme
- 12-18 July 2007, Schroecken, Vorarlberg, Austria
- Lectures of the MiPsummer School on "Mitochondrial Respiratory Phyisology" will provide a balance between an introduction into the basic concepts, advanced methodological and scientific approaches, and specific applications, with a focus on mitochondrial respiratory function linked to the general theme of MiP.
- For addresses of lecturers, see MiPsummer Participants.
- Day 1: Mitochondrial respiratory capacity, coupling and respiratory control. Programme 1 - Abstracts 1
- Day 2: Basic science and clinical reality 1. Programme 2 - Abstracts 2
- Day 3: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide. Programme 3 - Abstracts 3
- Day 4: Mitochondrial membrane potential. Programme 4 - Abstracts 4
- Day 5: Basic science and clinical reality 2. Programme 5 - Abstracts 5
1 - Friday, 13. July
- MiPsummer Morning Lectures. Mitochondrial respiratory capacity, coupling and respiratory control - Abstracts Day 1
- Chair: Steven Hand (US), Susana Cadenas (ES)
1L-01 | 09:00-10:00 | Erich Gnaiger (Innsbruck, AT) MitoPathways and mitochondrial respiration in top gear – an introduction to the concept of the ‘Q-junction’ and respiratory control with multiple substrates.
Reading: Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp.21-37. |
1L-02 | 10:00-11:00 | Erich Gnaiger (Innsbruck, AT) P/O, respiratory and uncoupling control ratios – established concepts re-visited.
Reading: Gnaiger E (2001) Respir. Physiol. 128: 277-297. |
11:00 | Coffee | |
1L-03 | 11:45-12:45 | Kathrin Renner (Regensburg, DE) Development of a respiratory protocol to detect mitochondrial defects under drug treatment.
Reading: Renner et al (2003) Biochim. Biophys. Acta 1642: 115-123. |
13:00 | Lunch | |
MiPsummer Afternoon Workshop: Permeabilized muscle fibers and high-resolution respirometry | ||
1W | 15:00-19:00 | Hélène Lemieux, Erich Gnaiger (Innsbruck, AT)
Permeabilized fiber preparation: Mouse heart. Multiple substrate/inhibitor protocol: A lecture hall experiment on-line. Cytochrome c oxidase inhibitor titration – biochemical threshold and excess capacity. |
17:00-18:15 | Coffee and Posters | |
Posters: | ||
1P-01 | Marco Alves, Paulo J Oliveira, Rui A Carvalho (PT) Preservation of cardiac mitochondrial function during ischemia and ischemia-reperfusion: role of the cardioplegic protection by Celsior and Histidine buffer solution. | |
1P-02 | Dominique-Marie Votion (BE), Hélène Lemieux, Ange Mouithys-Mickalad, Didier Serteyn, Erich Gnaiger. Study of the mitochondrial respiration in the equine muscle with high-resolution respirometry: feasibility, preliminary results and potential applications. | |
1L-04 | 18:15-19:00 | Hélène Lemieux (Innsbruck, AT) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart.
Reading: Lemieux H, Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp. 38-42. |
20:00 | Dinner at Hotel Mohnenfluh' |
2 - Saturday, 14. July
- MiPsummer Morning Lectures. Basic science and clinical reality
- Chair: Kathrin Renner (DE), Erich Gnaiger (AT)
2L-01 | 09:00-10:00 | Hong Kyu Lee (Seoul, KR) The metabolic syndrome and mitochondrial function - towards an international mtDNA cybrid study group. |
2L-02 | 10:00-11:00 | Jaap Keijer (Wageningen, NL) Bioactive food components and energy metabolism. |
11:00 | Coffee | |
2L-03 | 11:45-12:45 | Vilma Borutaite (Kaunas, LT) Mitochondrial regulation of cell death: NO and cytochrome c redox state.
Reading: Mander P et al.(2005) J. Neurosci. Res. 79: 208-215. |
13:00 | Lunch | |
MiPsummer Special Topic: Basic science and clinical reality | ||
2L-04 | 15:00-15:45 | Hong Kyu Lee (Seoul, KR) Diabetes 2 and the role of mitochondrial pathophysiology.
Flash presentations of posters: |
2P-01 | 15:50-16:00 | Sandra Amaral, Luís Martins, Paula C. Mota, Paulo J. Oliveira, João Ramalho-Santos (PT) Mitochondrial testicular function: Effects of age and diabetes |
2P-02 | 16:00-16:10 | Timothy R Koves, Noland RC, Slentz DS, Ilkayeva O, Newgard CB, Muoio DM (US) Mitochondrial dysfunction and metabolic inflexibility in models of aging and diet-induced obesity. |
2P-03 | 16:10-16:20 | Joris Hoeks, JJ Briedé, J de Vogel, G Schaart, M Nabben, E Moonen-Kornips, MKC Hesselink, P Schrauwen (NL) Mitochondrial function, -content and -ROS production in rat skeletal muscle: effect of high-fat feeding. |
2P-04 | 16:20-16:30 | Maxi Meissner, G Henk Visser, Frans Stellaard, F Kuipers (NL) Bile acid signaling, FXR and energy expenditure – novel perspectives for the treatment of metabolic diseases. |
16:30–19:00 | Coffee and Posters | |
2P-05 | Ruwen Böhm, L Schild, Th Herdegen (DE) Different approaches to assessing mitochondrial function and their utility and accuracy for pharmaceutical testing. | |
2P-06 | Peter Lindinger, Martine Calame, Matthias Hoch, Alex N Eberle, Ralph Peterli, Thomas Peters, Sjouke Hoving, Hans Voshol, Ian Fearnley, John E Walker (CH) Proteome analysis of mitochondria in adipose tissues of Bariatric patients: Identification of risk factors and/or predictors for the treatment outcome. | |
2P-07 | Alena Čížková, Stránecký V, Ivánek R, Hartmannová H, Nosková L, Piherová L, Tesařová M, Hansíková H, Honzík T, Zeman J, Divina P, Paul J, Houštěk J, Kmoch S (CZ) h-MitoArray and gene expression analysis in 13 patients with mitochondrial ATP synthase deficiency. | |
2P-08 | Daniela Fornuskova, Stiburek L, Pejznochova M, Zeman J (CZ) Depletion of COX5A subunit in HEK293 cells. | |
2P-09 | Virginie Rhein, G Baysang, F Meier, L Ozmen, H Bluethmann, E Savaskan, F Müller-Spahn, C Czech, J Götz, A Eckert (CH) Changes in mitochondrial respiration in brain cells of transgenic mice models of Alzheimer’s disease | |
2P-10 | Olga Brantova, J Sladkova, H Hansikova, J Zeman (CZ) Fibroblasts cell culture as model for apoptosis detection. | |
2P-11 | Karin Eberhart, Ireen Ritter, Birgit Timischl, Reinhard Kofler, Peter Oefner, Kathrin Renner (DE) Synergistic effect of glucocorticoids and 2-deoxyglucose - energy metabolism as a new target in the treatment of leukemia. | |
2P-12 | Zdeněk Paris, Julius Lukeš (CZ) Functional analysis of genes encoding mitochondrial proteins in Trypanosoma brucei. | |
20:00 | Dinner at Hotel Mohnenfluh |
3 - Sunday, 15. July
- MiPsummer Morning Lectures: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide
- Chair: Vilma Borutaite (LT), Jaap Keijer (NL)
3L-01 | 09:00-10:00 | Erich Gnaiger (Innsbruck, AT) Respiratory control by oxygen pressure under normoxia and hypoxia.
Reading: Gnaiger E (2003) Adv. Exp. Med. Biol. 543: 39-55. |
3L-02 | 10:00-11:00 | Alessandro Giuffre (Rome, IT) Inhibition of respiratory oxidases by NO: Mechanistic and physiological aspects.
Reading: Brunori M et al (2006) Biochim. Biophys. Acta. 1757:1144-1154. |
11:00 | Coffee | |
3L-03 | 11:45-12:45 | Susana Cadenas (Madrid, ES) Inhibition of cellular respiration by edogenous nitric oxide and relative sensitivity of the main two cellular targets of nitric oxide.
Reading: Rodríguez-Juárez et al (2007) Biochem. J. 405: 223–231. |
13:00 | Lunch | |
MiPsummer Afternoon Workshop: Spectrophotometry and oxygen kinetics. – NO and kinetic models. | ||
3W | 15:00-17:00 | Natascha Sommer(DE), Francesca Scandurra, Thomas Derfuss, Erich Gnaiger. Simultaneous remission spectrophotometry and high-resolution respirometry. Respiratory control by oxygen and redox states of mitochondrial cytochromes in cells: Instrument demonstration. |
17:00-18:00 | Coffee and Posters | |
3P-01 | Enara Aguirre, F Rodríguez-Juárez, E Gnaiger, S Cadenas (ES) Inhibition of cellular respiration by nitric oxide during normoxia and hypoxia. | |
3P-02 | Felix Rodríguez-Juárez, E Aguirre, S Cadenas (ES) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration. | |
3D | 18:00-18:30 | Discussion: Oxygen kinetics, NO and models of competitive and non-competitive inhibition. |
19:00 | Dinner at Hotel Mohnenfluh | |
20:15 | Open air folk music – garden of Hotel Mohnenfluh. |
4 - Monday, 16. July
- MiPsummer Morning Lectures: Mitochondrial membrane potential and respiratory control.
- Chair: Rodrigue Rossignol (FR), Alessandro Giuffre (IT)
4L-01 | 09:00-10:00 | Vilma Borutaite (Kaunas, LT) Mitochondrial membrane potential and respiratory control. |
4L-02 | 10:00-11:00 | Steven C Hand (Baton Rouge, US) Mitochondrial membrane potential, pH gradients, and redox state.
Reading: Menze MA et al (2005) Am. J. Physiol. Regul. Integr. Comp. Physiol. 289: R68–R76. |
11:00 | Coffee and Posters | |
Posters: | ||
4P-01 | Magdalena Labieniec, Cezary Watala, Dominika Malinska (PL) Resorcylidene aminoguanidine (RAG) affects calcium mobilization and transmembrane potential in isolated rat liver mitochondria without impairments of respiratory chain.
Flash presentations and posters: | |
4P-02 | 11:30-11:40 | Dominika Malińska, Adam Szewczyk (PL) Mitochondrial potassium channel opener BMS-191095l – cytoprotective properties and the influence on mitochondrial physiology. |
4P-03 | 11:40-11:50 | David Julian, Allman Rollins (US) Mitochondrial movement is by Brownian motion in coelomocytes from an annelid worm.
In case of favourable weather conditions: |
12:00 | MiPsummer Walk to the cheese and wine reception at the Alpmuseum uf m Tannberg; refreshment in the lake Körebersee or at Hotel Körbersee.' | |
18:30 | MiPsummer Grill Party. | |
21:00 | Meeting of the MiPboard – MiP2008 (round table conference), MiP2009 (joint conference with UMDF), future perspectives (open for all participants). |
5 - Tuesday, 17. July
- MiPsummer Morning Lectures: Basic science and clinical reality
- Chair: Hong Kyu Lee (KR), Dominique-Marie Votion (BE)
5L-01 | 09:00-10:00 | Rodrigue Rossignol (Bordeaux, FR) Mitochondrial bioenergetics and structural network organization. |
5L-02 | 10:00-11:00 | Enrico Calzia(Ulm, DE) Small and large animal models of sepsis and I/R-injury - how to combine basic science and clinical reality. |
11:00 | Coffee | |
5L-03 | 11:45-12:45 | Charles Hoppel (Cleveland, US) Integrated mitochondrial function and the clinical utility of polarographic analysis. |
13:00 | Lunch | |
15:00 | MiPsummer Afternoon Workshop: Lectures on open topics; Round table discussion; Summary - evaluation | |
20:00 | MiPsummer Dinner at Hotel Mohnenfluh | |
5A | 22:00 | Charles Hoppel (Cleveland, US) Inner mitochondrial membrane and outer space. |