From Bioblast
MiPNet22.04 IOC120 Barcelona ES
Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry IOC120. »ES Barcelona Zorzano A |
OROBOROS (2017-03-20) Mitochondr Physiol Network
Abstract:
O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - satellite to MITOEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, ES Barcelona Zorzano A
Programme
Monday 20th | Topic | Weblink |
08:45 | Registration | |
09:00-09:15 | Welcome by Prof. Antonio Zorzano. | |
09:15-09:30 | Introduction of participants and their research interests. | IOC120 |
09:30-10:00 | Get started with the O2k: Overview with video clips. | O2k-Manual |
10:00-11:30 | Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay. | The Blue Book - SUIT reference protocol |
11:30-12:00 | Coffee/tea break | |
12:00-13:00 | Principles of high-resolution respirometry: From switching
on the O2k to the experimental result – oxygen sensor calibration and quality control. |
POS-calibration-SOP |
13:00-14:00 | O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol. | O2k-Fluorometry |
14:00-15:00 | Lunch | |
15:00-17:30 | Demo-Experiment: HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. | RP1 - RP2 |
17:30-18:30 | Protocol driver for Marks and data analysis. | |
18:30-20:00 | The Bioblast wiki, O2k-Network and feedback discussion. |
Venue
- Barcelona Science Park
- Room 1 Tower D
- R+D+I Towers building
- C/ Baldiri Reixac 4-8
- 08028 Barcelona
- To get to the workhsop location following routes are possible:
- Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination
- Another option is to take a bus(see picture below with bus plan)
- To get to the workhsop location following routes are possible:
- Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min
- Taxi will take about 20 min and the costs are approximately 20-25 €
- Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial
General information
- O2k-Fluorometer demo experiment: high-resolution respirometry and H2O2 production (Amplex red)
- Followed by the Barcelona MITOEAGLE Conference, MG and WC Meeting.
Registration
- Please register here for the O2k-Workshop.
- Registration deadline: March 13
Lecturers and tutors
Preliminary list of participants
Participant Institution Antje Garten UK_Birmingham_Manjeri_G: Institute of Metabolism and Systems Research, University of Birmingham (UK) Georgina Alexandra Rivera-Ingraham FR_Montpellier_Rivera Ingraham G: University of Barcelona (ES) Laura Herrero Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES) Pau Gama ES Barcelona Garcia-Roves PM : Physiological Science Department, University of Barcelona (ES) Alexander Lyakhovich Research Institute VHIR, Barcelona (ES) David Sebastián ES_Barcelona_Zorzano A: IRB Barcelona (ES) Sebastián Zagmutt Caroca University of Barcelona (ES) Matteo Giovarelli IT_Milan_Clementi_E: Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT) Rubén Martín Cabezuelo Lara Nogueras ES_Lleida_Boada J: IRB Lleida Hugo Gonzalo ES_Lleida_Boada J: IRB Lleida
Contact
- OROBOROS INSTRUMENTS
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2017, Next, O2k-Network